Table 1. Primer sequences for real-time PCR of equine tendon cDNA.

Gene	Direction	Primer sequence (5’–3’)	Anneal (°C)	Product (bp)
ACAN	Forward	TCA GGT ATC CCA TCC ACT TGC C	57	105
	Reverse	CGT CGT AGG TCT CAT TGG TGT CAC	57	105
ADAMTS4	Forward	ATG TGG TCA CTA TTC CTG CGG G	58	137
	Reverse	AGG GCA TCA GCG TGT ATT CAC C	58	137
ADAMTS5	Forward	TTA CGA GAG AGG ATT TAT GTG GGC	56	217
	Reverse	CGC TTA TCT TCT GTG GAA CCA AAG	56	217
BGN	Forward	TGA TTG AGA ACG GGA GCC TGA G	56	143
	Reverse	TTT GGT GAT GTT GTT GGT GTG C	56	143
COL1A1	Forward	CCT GGC AAG AAC GGA GAT GAT G	59	140
	Reverse	CCA CTG AAA CCT CTG TGT CCC TTC	59	140
COL2A1	Forward	ACT TTC CAA TCC CAG TCA CGC	56	129
	Reverse	GAT TCT CTG GAC TTG ACC CGT CTG	56	129
COL3A1	Forward	GGA AGT TGC TGA AGG AGG ATG C	55	166
	Reverse	TGG AAT CTC TGG GTT GGG ACA G	55	166
COMP	Forward	GCA AAC AAT GAA CAG CGA CCC	56	129
	Reverse	TGG TAG CCA AAG ATG AAG CCC	56	129
DCN	Forward	CCA AAG TGC GAA AGT CTG TGT TC	54	138
	Reverse	CAG CAA TGC GGA TGT AGG AGA G	54	138
FMOD	Forward	GCT CCA TCT TGA CCA CAA CCA G	55	123
	Reverse	CCT TTC ATA GAA CTG CCC ACT TCC	55	123
LUM	Forward	AGG TGC GTT TAC TTT CCT CTT GG	55	239
	Reverse	GGT CAA TCT GGT TAT TCC GAA GG	55	239
MMP3	Forward	ATT CTG CTG TTA CTA TGC GTG GC	55	218
	Reverse	TTT CCT GTC ACC TTC AAC CCC	55	218
MMP14	Forward	ACC AGG TGA TGG ATG GAT ACC C	56	126
	Reverse	CCC AGT GCT TGT CTC CTT TGA AG	56	126
TIMP1	Forward	GGA TAC TTC CAC AGG TCG GAG AAC	59	234
	Reverse	CGT CCA CAA GCA ATC AGT GTC AC	59	234
TIMP2	Forward	ACT CTG GCA ACG ACA TCT ACG G	57	261
	Reverse	TCT TCT TCT GGG TGG CAC TCA G	57	261
TIMP3	Forward	AGG GGC TGA ACT ATC GGT ATC ACC	59	242
	Reverse	TGC TCA AGG GTC TGT GGC ATT G	59	242
VCAN	Forward	CCC ATC TCA CAA GCA TCC TGT C	55	123
	Reverse	TGC CAT CAG TCC AAC GGA AG	55	123

Primers were designed by MacVector 11.1.