Table 2. Mixed regression modelling of gene expression data.
| Covariates | |||||
|---|---|---|---|---|---|
| Gene | P for model | Transection surgery | SideLateral (L) Medial (M) | Proximity to C or MCP | Distance from midmetacarpus /lesion site |
| Controls only | |||||
| ACAN | 0.021 | nd (0.38) | nd (0.22) | Increase (0.006) | |
| COL1A1 | 0.002 | nd (0.07) | nd (0.09) | Decrease (0.004) | |
| COL2A1 | <0.001 | nd (0.30) | nd (0.29) | Increase (<.001) | |
| COL3A1 | 0.005 | nd (0.14) | nd (0.17) | Decrease (0.004) | |
| MMP3 | 0.003 | nd (0.10) | MCP > C (0.001) | nd (0.91) | |
| ADAMTS4 | <0.001 | L > M (<.001) | C > MCP (0.033) | nd (0.35) | |
| ADAMTS5 | 0.005 | L > M (0.001) | nd (0.37) | nd (0.52) | |
| Controls and operated | |||||
| ACAN | 0.002 | Increase (<.001) | nd (0.26) | nd (0.22) | nd (0.22) |
| VCAN | <.001 | Increase (<.001) | L > M (0.002) | C > MCP (0.010) | nd (0.63) |
| BGN | 0.004 | Increase (0.001) | nd (0.23) | nd (0.79) | nd (0.12) |
| FMOD | 0.066 | - (0.011) | nd (0.19) | nd (0.47) | nd (0.93) |
| LUM | 0.012 | Increase (0.005) | L > M (0.038) | nd (0.75) | nd (0.34) |
| DCN | 0.11 | nd (0.06) | nd (0.49) | nd (0.67) | nd (0.08) |
| COMP | 0.72 | nd (0.58) | nd (0.94) | nd (0.97) | nd (0.19) |
| COL1A1 | <.001 | Increase (<.001) | L > M (<.001) | nd (0.66) | Decrease (<.001) |
| COL2A1 | <.001 | Increase (0.004) | M > L (<.001) | MCP > C (0.003) | Increase (<.001) |
| COL3A1 | <.001 | Increase (<.001) | L > M (0.001) | nd (0.40) | Decrease (<.001) |
| MMP3 | <.001 | Decrease (<.001) | nd (0.75) | MCP > C (<.001) | Increase (0.001) |
| MMP14 | <.001 | Increase (0.016) | L > M (<.001) | C > MCP (0.008) | Decrease (<.001) |
| ADAMTS4 | <.001 | Decrease (<.001) | L > M (<.001) | C > MCP (<.001) | Decrease (<.001) |
| ADAMTS5 | <.001 | nd (0.29) | L > M (<.001) | C > MCP (0.026) | nd (0.49) |
| TIMP1 | <.001 | Increase (0.019) | L > M (<.001) | nd (0.05) | nd (0.06) |
| TIMP2 | 0.29 | nd (0.36) | nd (0.24) | nd (0.10) | nd (0.92) |
| TIMP3 | 0.002 | Decrease (<.001) | nd (0.95) | nd (0.62) | nd (0.06) |
| GAPDH | <.001 | nd (0.66) | L > M (<.001) | C > MCP (0.007) | nd (0.75) |
Data was clustered by horse, limb and proximity to carpus (C) or metacarpophalanges (MCP). Significant effects of surgery and SDFT spatial position as covariates (and resultant P values) were obtained with all indicated covariates included in the model. Results are given for significant models without transection (n = 72; controls only) and from all models with transection (n = 140). nd = no difference; C = carpus; MCP = metacarpophalangeal joint.