Skip to main content
. Author manuscript; available in PMC: 2015 Oct 2.
Published in final edited form as: Nature. 2015 Jan 19;520(7545):94–98. doi: 10.1038/nature14051

Fig. ED7. Role of PKA and cAMP in the α-MSH-induced closure of Kir7.1.

Fig. ED7

a & b, Subtracted Tl+ flux assay examining effects of 100 nM α-MSH indicate that pre incubation with 1 μM H89, a PKA inhibitor, fails to block α-MSH -induced regulation of Kir7.1. Data show (a) mean +/−sem of kinetic traces, and (b) maxima (n=16). VHC = vehicle. c & d, Subtracted Tl+ flux assay examining effects of raising intracellular cAMP by forskolin (FSK, 20 μM) and IBMX treatment, with and without 100 nM α-MSH. Data show (c) kinetic traces, and (d) maxima. (IBMX: 100μM 3-isobutyl-1-methylxanthine, VHC: vehicle, mean +/− SEM, n=64, ***p<0.01, unpaired t test).