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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Clin Cancer Res. 2014 Oct 29;21(7):1722–1733. doi: 10.1158/1078-0432.CCR-14-2022

Figure 4. Pharmacological inhibition of the endocytic pathway blocks exosome uptake into INS-1 cells.

Figure 4

(A) INS-1 cells were treated with varying concentrations of Amiloride (25, 50, and 100 µM) for 15 minutes. PANC-1 exosomes were dyed with PKH67 and incubated with the cells after Amiloride treatment for 5 hours to allow exosome uptake. Scale bars represent 10 µm. (B) Zoom images (10.5X) of exosome internalization into INS-1 cells in the presence or absence of 100 µM Amiloride. Scale bar represents 5 µm. (C) Quantification of green fluorescence/cell in all treatment groups. (D) INS-1 cells were treated with 25 µg/ml or 50 µg/ml Nystatin for 30 minutes. PKH67-dyed exosomes were incubated with the cells for 5 hours to allow internalization. Scale bars represent 10 µm. (E) Zoom images (10.5X) of cells treated with 50 µg/ml Nystatin compared to control (untreated) cells show decreased PKH67-dyed exosome internalization. Scale bar represents 5 µm. (F) Green fluorescence quantification of internalized exosomes in the presence of absence of Nystatin. ***, P<.0001.