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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Cancer Res. 2015 Feb 17;75(7):1388–1398. doi: 10.1158/0008-5472.CAN-14-2561

Figure 6. Knockdown of 15-PGDH prevents Fat-1-induced inhibition of cholangiocarcinoma growth.

Figure 6

Fat-1 overexpressed or control CCLP1 cells were transfected with 15-PGDH siRNA and the cells were evaluated for proliferation (WST-1) and colony formation. (A) The levels of 15-PGDH protein in cells with or without 15-PGDH knockdown. The cells were lysed 72 h after 15-PGDH siRNA transfection; total protein was analyzed by western blot with 15-PGDH antibody (β-actin was measured as a reference gene). (B) Knockdown 15-PGDH enhances CCLP1 cell growth and prevents Fat-1 induced inhibition of growth. The data are presented as mean±SE from 3 independent experiments. *P<0.05; **P<0.01; (C) Knockdown of 15-PGDH in CCLP1 cells prevents Fat-1 induced inhibition of colony formation. Representative of three independent experiments were showed in the left panel. Quantified results were normalized to vector control group and presented as mean±SE in the right panel; *P<0.05; **P<0.01.