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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Cancer Res. 2015 Feb 3;75(7):1366–1375. doi: 10.1158/0008-5472.CAN-14-1849

Fig. 3. Bcl-2 interacts with multiple pro-apoptotic Bcl-2 family proteins in RS4;11 cells.

Fig. 3

A. Bcl-2 was immunoprecipitated from RS4;11 cells, and subjected to immunoblotting for Bcl-2 and the indicated pro-apoptotic Bcl-2 family members. WCE, whole cell extract; Sup, supernatant; IP, immunoprecipitate; eluant, detergent eluant of immunoprecipitate. Samples were obtained from immunoprecipitations performed in the absence (−) or presence (+) of Bcl-2 antibody. Asterisks indicate non-specific bands. B. Bim was immunoprecipitated from RS4;11 cells, and subjected to immunoblotting for Bim and Bcl-2. C. Bik was immunoprecipitated from RS4;11 cells, and subjected to immunoblotting for Bik and Bcl-2.