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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Cancer Res. 2015 Feb 3;75(7):1366–1375. doi: 10.1158/0008-5472.CAN-14-1849

Fig. 4. Bcl-2 interacts with multiple pro-apoptotic Bcl-2 family proteins in primary ALL cells.

Fig. 4

A. Bcl-2 was immunoprecipitated from ALL-19 cells and subjected to immunoblotting for Bcl-2 and the indicated pro-apoptotic Bcl-2 family members. WCE, whole cell extract; Sup, supernatant; IP, immunoprecipitate; eluant, detergent eluant of immunoprecipitate. Samples were obtained from immunoprecipitations performed in the absence (−) or presence (+) of Bcl-2 antibody. Asterisks indicate non-specific bands. B, C, and D. Bax (panel B), Bim (panel C) or Bik (panel D) were immunoprecipitated from ALL-19 cells, and subjected to immunoblotting for the proteins indicated.