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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Cancer Res. 2015 Feb 3;75(7):1366–1375. doi: 10.1158/0008-5472.CAN-14-1849

Fig. 6. Leukemic blasts from pediatric ALL patients express high levels of Bcl-2 and are highly sensitive to ABT-263.

Fig. 6

Blasts from two patients, termed P1-ALL and P2-ALL, were isolated and cultured, as described in Materials and Methods. A. Extracts were made from KB3 or RS4;11 cell lines or from P1-ALL and P2-ALL cultures as indicated and subjected to immunoblotting for Bcl-2, Bcl-xL or Mcl-1. GAPDH was used as a loading control. B, C. Cell viability was assessed by MTT assay as described in Materials and Methods. P1-ALL or P2-ALL cells as indicated were treated with vehicle (100% viability) or increasing concentrations of ABT-263 for 72 h. Results are given as mean ± s.d. (n = 6).