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. Author manuscript; available in PMC: 2016 Apr 6.
Published in final edited form as: J Proteomics. 2014 Oct 2;118:81–94. doi: 10.1016/j.jprot.2014.09.011

Figure 7.

Figure 7

MED4 localizes to the centrosome throughout the cell cycle. (A) BioID and AP-MS analyses of MED4 reveal significant association with centrosomal proteins. Highlighted box #2 from the heat map presented in Figure 6 is shown. (B) BirA*-FLAG tagged MED4 shows strong immunofluorescence staining consistent with centrosomal localization in interphase cells (top panels). BirA*-FLAG tagged MED23 is only observed in the nucleus but not at centrosomes (bottom panels). 3XFLAG-MED4 co-localizes with two centrosomal markers, pericentrin (C) and centrin (D), by immunofluorescence in interphase cells (top panels) and in cells undergoing division (bottom panels).