Fig. 2.

Gene expression in the inflamed murine intestine. a TNF-α and IL-1β mRNA expression and b NHE3, PDZK1, and NHERF1 mRNA expression in distal ileum or mid-colon in the three mouse models as described in the text. a The proinflammatory cytokines were elevated in the inflamed vs control intestinal mucosa, but the pattern of cytokine increase was different in the different models both qualitatively and quantitatively. b Nevertheless, a significant decrease in PDZK1 mRNA expression was observed in the inflamed distal ileum of Tnf ^ΔARE+/− and mid-colon of Il-10 ^−/− and Rag2 ^−/− CD4⁺ CD45^RBhigh mice compared to their respective controls. NHE3 mRNA was increased in the fairly acute Rag2 ^−/− CD4⁺ CD45^RBhigh transfer colitis mouse model. Bar graphs are represented as mean ± SEM. n = 5–6 pairs of mice. *P < 0.05, **P < 0.005, ***P < 0.0005