Fig. 6.

NHE3 activity measurements in the inflamed murine intestine. Acid-activated NHE3 activity was significantly decreased compared to the respective controls, a in the isolated ileal villi of Tnf ^ΔARE+/− mice, b in isolated colonic crypts (10–15 crypts in each mice) from Il-10 ^−/−, and from c Rag2 ^−/− CD4⁺ CD45^RBhigh mice. 20 μM of S1611 inhibited NHE3 activity even in Tnf ^ΔARE+/− and Rag2 ^−/− CD4⁺ CD45^RBhigh mice, which shows that NHE3 activity was not completely lost in inflamed enterocytes (data not shown). A total of three to five pairs of inflamed and control mice were used for the experiments. Bar graphs are represented as mean ± SEM. *P < 0.05 and **P < 0.005