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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Mol Neurobiol. 2014 Oct 2;52(1):679–695. doi: 10.1007/s12035-014-8881-8

Figure 5.

Figure 5

The IRE1 signaling pathway of the UPR is strongly activated in photoreceptors of RhoP23H/+ERAI+/− mice. (A–H) Rhodopsin (red) and XBP1-Venus (by anti-GFP, green) were visualized in eyes from RhoP23H/+ERAI+/− mice and Rho+/+ERAI+/− mice at P30. DAPI (blue) highlights photoreceptor nuclei. Strong XBP1-Venus staining is visualized in the IS and perinuclear region of ONL photoreceptors expressing P23H rhodopsin. (I–L) Peanut agglutinin (PNA) staining of cones (red) and XBP1-Venus (by anti-GFP, green) were visualized in eyes from RhoP23H/+ERAI+/− mice and Rho+/+ERAI+/− mice at P120. DAPI (blue) highlights photoreceptor nuclei. White arrow in (L) indicated the cone cell overlapping with XBP1-Venus signal. (M) XBP1-Venus (by anti-flag) protein levels were detected in retinal lysates from RhoP23H/+ERAI+/− mice and littermate control Rho+/+ERAI+/− mice at P30. HSP90 served as a protein loading control. (N) XBP1-Venus protein levels were detected by anti-flag or anti-GFP in retinal lysates from RhoP23H/+ERAI+/− mice at indicated ages.