Z-stack reconstruction of a cell plated on 100 kPa (a, c) or 400 kPa (b, d) at 3 days after plating. NRVM fixed and stained for myofibril structure (alpha-actinin, red), focal adhesions (paxillin, green) and nucleus (DAPI, blue). Posts are black holes in the upper right region of the cells. (e) NRVM is capable of displacing a 100 kPa micropost, arrows. (f) NRVM does not displace 400 kPa microposts, arrow. (a–d) Distance scale = 20 µm. (e, f) Distance scale = 15 µm, Time Scale = 1 s.