Fig. 2.

Glaucine inhibits the migration and invasion of PMA-treated MCF-7 cells. a PMA-induced MCF-7 cells were scratched with a pipette tip and incubated with the indicated concentration of glaucine for 36 h. The wound area was quantified by measuring the cell-free area. Original magnification was × 100. Scale bars, 100 μm. Cell migration into the wound area is represented as the percentage of recovery relative to 0 h. ^# P < 0.01 versus vehicle alone-treated cells, ^* P < 0.01 versus PMA alone-treated cells. b MCF-7 cells were incubated with 100-nM PMA and the indicated concentration of glaucine for 36 h. The invasion ability of MCF-7 cells was determined by a Matrigel invasion assay. Original magnification was × 200. Scale bars, 100 μm. The cell invasion ability was quantified; ^# P < 0.01 versus vehicle alone-treated cells, ^* P < 0.01 versus PMA alone-treated cells. Data represent the mean ± SE of three independent experiments