Table 1.
The effects of acute administration of salsolinol on l-DOPA-induced changes on dopamine metabolism in rat brain structures
| Treatment | DA (ng/g tissue) | DOPAC (ng/g tissue) | HVA (ng/g tissue) | [HVA]/[DA] × 100 | |
|---|---|---|---|---|---|
| Acute | Acute | ||||
| Substantia nigra | |||||
| Saline | Saline | 889 ± 51 | 212 ± 34 | 106 ± 15 | 12 ± 2 |
| Saline | l-DOPA 100 | 3985 ± 687** | 12,888 ± 2632** | 6208 ± 1000** | 187 ± 39** |
| Salsolinol 100 | Saline | 773 ± 41 | 185 ± 14 | 102 ± 7 | 13 ± 0.8 |
| Salsolinol 100 | l-DOPA 100 | 4146 ± 724** | 13,981 ± 1066** | 5841 ± 403** | 188 ± 57** |
| Effect of salsolinol |
F (1/27) = 0.001 N.S. |
F (1/27) = 0.05 N.S. |
F (1/27) = 0.04 N.S. |
F (1/27) = 0.001 N.S. |
|
| Effect of l-DOPA |
F (1/27) = 21.85 P < 0.01 |
F (1/27) = 29.4 P < 0.01 |
F (1/27) = 40.8 P < 0.01 |
F (1/27) = 16.9 P < 0.01 |
|
| Interaction of salsolinol + l-DOPA |
F (1/27) = 0.04 N.S. |
F (1/27) = 0.05 N.S. |
F (1/27) = 0.04 N.S. |
F (1/27) = 0.001 N.S. |
|
| Striatum | |||||
| Saline | Saline | 9043 ± 430 | 1238 ± 25 | 675 ± 46 | 7.4 ± 0.3 |
| Saline | l-DOPA 100 | 17,802 ± 1967* | 19,266 ± 3690** | 10,057 ± 1587** | 57 ± 7.9** |
| Salsolinol 100 | Saline | 9457 ± 411 | 1573 ± 109 | 1017 ± 83 | 11 ± 0.5 |
| Salsolinol 100 | l-DOPA 100 | 19,351 ± 3151** | 22,534 ± 2433** | 10,323 ± 503** | 62 ± 12** |
| Effect of salsolinol |
F (1/27) = 0.2 N.S. |
F (1/27) = 0.3 N.S. |
F (1/27) = 0.4 N.S. |
F (1/27) = 0.2 N.S. |
|
| Effect of l-DOPA |
F (1/27) = 17.8 P < 0.01 |
F (1/27) = 30.9 P < 0.01 |
F (1/27) = 40.8 P < 0.01 |
F (1/27) = 29 P < 0.01 |
|
| Interaction of Salsolinol + l-DOPA |
F (1/27) = 0.07 N.S. |
F (1/27) = 0.2 N.S. |
F (1/27) = 0.001 N.S. |
F (1/27) = 0.01 N.S. |
|
Salsolinol (100 mg/kg i.p.) or l-DOPA (100 mg/kg i.p.) was acutely administered (100 mg/kg i.p.). In the combined treatment group, l-DOPA (100 mg/kg i.p.) was administered once 15 min after salsolinol administration. The rats were decapitated 2 h after injection. The concentration of dopamine and its metabolites were measured using HPLC. The results are expressed as the means ± SEM (n = 7–10 animals per group). The data were analyzed via two-way ANOVA followed by Duncan’s test. Statistical significance: * P < 0.05, ** P < 0.01 versus the control group; + P < 0.05, ++ P < 0.01 versus the l-DOPA group