Figure 6.
Read through compound treatment rescues MeCP2 expression in vitro. (A) Representative western blot demonstrating no full-length MeCP2 protein from MEFs derived from Mecp2R255X/Y mice after vehicle treatment, but full-length MeCP2 protein after 72 h treatment with 800 µg/ml gentamicin. Top panel was probed using an antibody against MeCP2, bottom panel probed with antibody against histone H3 (H3) as a loading control. (B) Quantitation of western blots as shown in (A) representing the three technical replicates examined per group.