Table 2. Overview of TrypanoCyc content before and after curation and comparison with the KEGG database.

Evidence type	Confidence score	Description
Biochemical data	4	Direct evidence for gene product function and biochemical reaction: protein purification, biochemical assays, experimentally solved protein structures and comparative gene-expression studies.
Genetic data	3	Direct and indirect evidence for gene function: knock-out characterization, knock-in characterization and over expression.
Physiological data	2	Indirect evidence for biochemical reaction based on physiological data: secretion products or defined medium components serve as evidence for transport and metabolic reactions.
Sequence data	2	Evidence for gene function: genome annotation, SEED annotation.
Modelling data	1	No evidence is available but reaction is required for modelling. The included function is a hypothesis and needs experimental verification. The reaction mechanism may be different from the included reaction(s).
Not evaluated	0
Negative hypothesis	–1	Although there is no evidence against this reaction, it is expected to not exist
Evidence against the reaction	–2	Direct/indirect evidence against the hypothesis is available