Table 1.
Kinetic parameters obtained from local fitting of the interaction between the immobilized HSA and HSA-specific antibody for each mathematical model. The average rate constant and standard error of the local fit values for each model are reported.
| Interaction Model |
ka1 [M−1s−1] |
kd1 [s−1] |
ka2 [M−1s−1] |
kd2 [s−1] |
χ2 [Pixel2] |
|---|---|---|---|---|---|
| 1:1 binding | 4 (± 1) ×104 |
2.1 (± 0.2) ×10−3 |
/ | / | 1.8 |
| heterogeneous analyte | 2.3 (± 0.9) ×104 |
1.2 (± 0.4) ×10−2 |
5 (± 2) ×104 |
7 (± 4) ×10−4 |
0.1 |
| bivalent analyte | 3.1 (± 0.7) ×104 |
1.8 (± 0.7) ×10−4 |
1.2 (± 0.7) ×106 [Pixel−1s−1] |
9 (± 5) ×10−3 |
0.8 |