Fig. 4. Confocal imaging of cholyl-lysyl-fluorescein (CLF) distribution within spheroids. Spheroids were treated with CLF for 30 minutes and images show evidence of the concentration of the fluorescent compound in these structures. Staining was punctate and did not correspond to a pan-cellular localisation within individual cells. This may reflect the presence, in part, of canalicular structures between cells from which bile release from hepatocyte spheroids is mediated. Spheroids used from 15 days post isolation. Images from left to right: fluorescence channel; Bright field scan; merged image. All scale bars indicate 100 μm.
