Table 1. PCR primers and product sizes.

Gene	Primer sequence	Product size (bp)	Primer concentration (pM)	Calculated average primer efficiency (%)	References
CYP1A	F: 5′-GAT GTC AGT GGC AGC TTT GA-3′	104	2.0	101.6	N/A
	R: 5′-TCC TGG TCA TCA TGG CTG TA-3′
CYP2K1	F: 5′-CTC ACA CCA CCA GCC GAG AT-3′	164	1.5	97.9	78
	R: 5′-CTT GAC AAA TCC TCC CTG CTC AT-3′
CYP2M1	F: 5′-GCT GTA TAT CAC ACT CAC CTG CTT TG-3′	195	1.5	97.2	78
	R: 5′-CCC CTA AGT GCT TTG CAT GTA TAG AT-3′
CYP3A27	F: 5′-GAC GGT GGA GAT CAA CG-3′	240	1.0	96.2	79
	R: 5′-GAG GAT CTC GAC CAT GG-3′
UGT	F: 5′-ATA AGG ACC GTC CCA TCG AG-3′	113	3.0	97.3	N/A
	R: 5′-ATC CAG TTG AGG TCG TGA GC-3′
MDR1	F: 5′-GGA ACT GTC CTC ACC GTG TT-3′	136	3.0	100.5	N/A
	R: 5′-GGG GTT TAT TGT CGG TGA TG-3′
MRP2	F: 5′-CCA TTC TGT TCG CTG TCT CA-3′	150	2.0	95.1	N/A
	R: 5′-CTC GTA GCA GGG TCT GGA AG-3′
BCRP	F: 5′-AGG CCT GCT GGT GAA CCT G-3′	101	4.0	95.8	46
	R: 5′-ACT CAT TAA TTT GGA GAG CTG TTA GTC C-3′
BSEP	F: 5′-CCG ACC AGG GCA AAG TGA TT-3′	101	1.5	98.6	N/A
	R: 5′-CAG AAT GGG CTC CTG GGA TAC-3′
18S rRNA	F: 5-TGG AGC CTG CGG CTT AAT TT-3′	170	2.0	97.4	46
	R: 5′-ATG CCG GAG TTT CGT TCG TT-3′