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ITC binding analysis of AGT-LRM and AGT-LRM-SKL proteins with Pex5p-pbd. Thermograms (A) and binding isotherms (B) at 25 °C for AGT-LRM, AGT-LRM-HMG and AGT-LRM-SKL; AGT-LRM (15 μM in subunit) was titrated using Pex5p-pbd 350 μM (29 injections of 1.2 μL) while AGT-LRM-HMG and AGT-LRM-SKL (20 μM in subunit) was titrated using Pex5p-pbd 250 μM (12–16 injections of 2–2.2 μL).
