Table 3.
Thermodynamic binding parameters for the interaction of Pex5p-pbd with AGT and AGT-LRM variants at pH 7.4 and 25 °C. Data are mean ± s.d. of three independent titrations.
| AGT Variant | N | Kd | ΔG (kJ/mol) | ΔH (kJ/mol) | -TΔS (kJ/mol) | ΔCpa (kJ/mol·K) |
|---|---|---|---|---|---|---|
| AGT | 1.0 ± 0.1 | 1.4 ± 0.2 μM | −33.5 ± 0.4 | 21.7 ± 3.7 | −55.2 ± 1.2 | −2.5 ± 0.4 |
| AGT-LRM | 1.1 ± 0.1 | 1.6 ± 0.4 μM | −33.0 ± 0.8 | 21.3 ± 0.4 | −55.3 ± 3.6 | −2.7 ± 0.2 |
| AGT-SKL | 0.8 ± 0.1 | 44 ± 10 nM | −43.1 ± 0.9 | 6.3 ± 0.8 | −49.4 ± 1.7 | −2.7 ± 0.1 |
| AGT-LRM-SKL | 0.9 ± 0.1 | 65 ± 27 nM | −41.0 ± 0.7 | 5.0 ± 0.3 | −46.0 ± 0.5 | −2.6 ± 0.1 |
a ΔCp values are determined from the linear dependence of ΔH on temperature.