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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Immunol. 2015 Jan 16;194(4):1894–1904. doi: 10.4049/jimmunol.1402377

Figure 8. Macrophages harvested from bleomycin challenged mice can regulate the profibrotic effector functions of fibroblasts.

Figure 8

Fibroblasts were cultured from previously unchallenged WT mice. Lung macrophages were collected from WT and IRAK-M−/− mice that were either unchallenged or day 14 post-bleomycin challenge. CD45+ cells were isolated from lung macrophages and total numbers of macrophages were quantified by modified Wright-giemsa stain. A. and B. Approximately 1 × 106 total macrophages were co-cultured with 5 × 105 WT fibroblasts. The expression of αSMA (A) and collagen III (B) were measured by realtime RT-PCR. C. Cell free supernatants were harvested from macrophages isolated from bleomycin challenged and cultured with WT unchallenged fibroblasts. The expression of collagen III was measure by realtime RT-PCR. D. Lung macrophages from unchallenged or bleomycin challenged mice were incubated with either control IgG or anti-IL-13 neutralizing antibody (2 µg/ml). The expression of pro-fibrotic mediators were measured after 48 h in culture by realtime RT-PCR (D). Graphs represent mean ± SEM with n=4 samples/group from 2 separate experiments. *p<0.05, **p<0.01, ***p<0.001 when compared to fibroblasts from WT unchallenged mice.