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. 2014 Dec 31;1(1):ENEURO.0055-14.2014. doi: 10.1523/ENEURO.0055-14.2014

Figure 7.

Figure 7

FMRP regulates dendritic development. In vivo two-photon time-lapse images of cells expressing Sox2bd::eGFP and CMO or fmr1a MO collected at 2 and 3 dfe. A, Two-photon Z-projections of imaged cells and their reconstructed dendritic arbors at 2 and 3 dfe for cells with FMRP knockdown compared to control. B, HIGH (0.1 mM) fmr1a MO decreased total dendritic length at 3 dfe (**p < 0.01). C, HIGH fmr1a MO decreased total dendritic branch tip number at 2 and 3 dfe (*p < 0.05, **p < 0.01). D, Branch density was unchanged between the groups. E, Two-photon Z-projection and reconstructed dendritic arbor of a cell expressing 1 μg/μl Δfmr1-t2A-eGFP (HIGH FMRP OE) at 3 dfe. F, G, HIGH FMRP OE decreased total dendritic length (F) and total dendritic branch tip number (G) compared to control (Sox2bd::eGFP; ***p < 0.001). H, Two-photon Z-projections of imaged cells and their reconstructed dendritic arbors at 3 dfe for cells when FMRP is knocked down (HIGH fmr1a MO), overexpressed with 0.5 μg/μl Δfmr1-t2A-eGFP (LOW FMRP OE), and rescued (HIGH MO LOW Δfmr1 Rescue) compared to control (CMO). I, J, Co-electroporation of LOW Δfmr1-t2A-eGFP rescued HIGH fmr1a MO-mediated decreases in total dendritic length (I) and dendritic branch tip number (J) (*p < 0.05, **p <0.01). Scale bars, 20 μm.