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. Author manuscript; available in PMC: 2015 Apr 3.
Published in final edited form as: Structure. 2012 Mar 7;20(3):545–553. doi: 10.1016/j.str.2012.01.018

Figure 6.

Figure 6

Mutational analysis of the nucleotide and metal-binding residues of Cmr2dHD. ReconstitutedCmr complexes (Cmr1-6, 1 μ M) containing no Cmr2 (-Cmr2), Cmr2dHD or mutants thereof (asindicated) were incubated with crRNAs (0.1 μ M) of 39 nt or 45 nt (as indicated) for 30 minutesbefore the addition of γ 32P-labeled target RNA (labeled by an open arrow). The reactionmixtures were incubated for 1 hour and analyzed on 15% denaturing polyacrylamide gel. – lanescontain no crRNA or proteins. Cleavage products are indicated by asterisks. Target RNA/crRNA duplexes are indicated by a closed arrow. See also Table S1.