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. Author manuscript; available in PMC: 2015 Apr 3.
Published in final edited form as: J Immunol. 2012 Mar 19;188(8):3839–3850. doi: 10.4049/jimmunol.1003875

FIGURE 1.

FIGURE 1

SHIP negatively regulates MC maturation, but positively regulates IgE-induced BMMC survival. SHIP+/+ and SHIP−/− CTMC, MMC, and BMMC cultures were initiated, as described in Materials and Methods, and (A) their maturity was assessed by surface expression of c-kit and FcεR1α. Cells that were doubly positive were considered mature. (B) Proliferation was assessed by counting trypan blue excluding cells. Shown is the absolute cell number divided by the starting number of cells at week 0. (A and B) SHIP +/+ MCs = ▲ with solid lines; SHIP−/− MCs = △ with dashed lines. (C) SHIP+/+ and SHIP−/− MC subsets were incubated in 0.1% BSA medium (SHIP+/+ = ▲; SHIP−/− = △, solid lines) or +5 μg/ml SPE-7 IgE (SHIP+/+ = #x025BC;; SHIP−/− = ▽, dashed lines), and viable cells were counted. Values are the mean ± SD from two independent experiments in triplicate. Significant differences (p < 0.05) are indicated (*).