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. Author manuscript; available in PMC: 2015 Apr 3.
Published in final edited form as: J Immunol. 2012 Mar 19;188(8):3839–3850. doi: 10.4049/jimmunol.1003875

FIGURE 2.

FIGURE 2

IgE plus Ag-induced degranulation of CTMCs, MMCs, and BMMCs is repressed by SHIP. (A) SHIP+/+ (black bars) and SHIP−/− (white bars) CTMCs, MMCs, and BMMCs were preloaded with 0.1 μg/ml IgE for 18 h at 37°C and exposed to the indicated concentration of Ag for 1 h at 37°C, and degranulation was assessed. (B) SHIP+/+ (black bars) and SHIP−/−(white bars) CTMCs, MMCs, and BMMCs were treated N/S, or exposed to 100 ng/ml LPS, 50 μg/ml dsRNA, 0.3 μM CpG, or 10 μg/ml PGN for 1 h, and degranulation was assessed. (C and D) MCs were preloaded with IgE, as in (A), and then either N/S or treated with TLR ligands [same concentrations as in (B)] plus 2 ng/ml Ag (B) or 0.2 ng/ml Ag (C) for 1 h. (E) SHIP+/+ (black bars) and SHIP−/−(white bars) BMMCs, MMCs, and CTMCs were treated with 10 μg/ml SPE-7 IgE or 400 ng/ml SCF for 1 h at 37°C, and degranulation was assessed. As well, as a positive control, SHIP+/+ (black bars) and SHIP−/−(white bars) CTMCs were preloaded with 0.1 μg/ml SPE-7 IgE for 18 h at 37°C and exposed to 20 ng/ml DNP-HSA for 1 h at 37°C, and degranulation was assessed. Data shown are the means ±SD from at least two independent experiments carried out in duplicate (A–C) or one experiment in triplicate (D) or duplicate (E). Similar results were obtained in at least two independent experiments.