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. Author manuscript; available in PMC: 2015 Apr 3.
Published in final edited form as: J Biol Chem. 2001 Jun 14;276(34):32136–32144. doi: 10.1074/jbc.M104580200

Table I.

Strains and plasmids used in this work

Strain Genotype
S. cerevisiae
 GYC86 MATa his3 leu2 trp1 ura3/MATα his3 leu2 trp1 ura3
 TCY57 Matα lys2 ura3 trp1::hisG leu2::hisG GAL1,10-URE2
 RR912 Matα lys2 ura3 gln3Δ::hisG trp1Δ::hisG his3Δ::hisG
 STCY32 MATa lys5 ura3 trp1Δ::hisG his3Δ::hisG
 BY4742 MATα his3D1 leu2D0 lys2D0 ura3D0
E. coli
 DH5α [F/endA1 hsdr17(rK mK+) supE44 thi1 recA1 Δ(lacZYA-argF)U169 (m80lacZΔ1M15)]