Table II.

Oligonucleotide primers and DNA fragments used for dal82Δ constructs

Primer	Sequence
T7–7	5'–GGG AGA CCA CAA CGG TTT CCC–3′ (T7 promoter)
RD21	5′–GGG CAT TGT GTG AAG AGT AGG–3′
RD22	5′–G GGG GAT CCC TGG CAA AGG GTG CTC GAT GAG–3′
RD24	5′–G GGG AAT TCG CCC CGG ATG ACT TTT AAT–3′
RD25	5′–G GGG AAT TCC TCC CAT CAG CTT CAA CTG–3′
RD37	AG CTT CAT CCA AGA CCA AGA ACT AAA TTC GGA AAT GAA TCA TCT TCA TCC    A GTA GGT TCT GGT TCT TGA TTT AAG CCT TTA CTT AGT AGA AGT AGG TTA TCA TCA TCT TCT TTC ATT AAA AGT CAT CCG GGG AAT AGT AGT AGA AGA AAG TAA TTT TCA GTA GGC CCC
RD38	AG CTT TTC GGA AAT GAA TCA TCT TCA TCC TTA TCA TCA TCT TCT TTC ATT AAA  A AAG CCT TTA CTT AGT AGA AGT AGG AAT AGT AGT AGA AGA AAG TAA TTT    AGT CAT CCG GGG    TCA GTA GGC CCC
RD39	AG CTT ATG GGA GCA CTC TTG GAC GAA GCA CCA GAA CAT CCA AGA CCA AGA ACT    A TAC CCT CGT GAG AAC CTC CTT CGT GGT CTT GTA GGT TCT GGT TCT TGA     AAA TTC GGA AAT GAA TCA TCT TCA TCC TTA TCA TCA TCT TCT     TTT AAG CCT TTA CTT AGT AGA AGT AGG AAT AGT AGT AGA AGA
RD40	AG CTT ATG GGA GCA CTC TTG GAC GAA GCA CCA GAA CAT CCA AGA CCA AGA ACT    A TAC CCT CGT GAG AAC CTC CTT CGT GGT CTT GTA GGT TCT GGT TCT TGA     AAA TTC GGA AAT GAA ATT AAA AGT CAT CCG GGG     TTT AAG CCT TTA CTT TAA TTT TCA GTA GGC CCC
RD41	AG CTT ATG GGA GCA CTC TTG GAC GAA GCA CCA GAA TTC GGA AAT GAA TCA TCT    A TAC CCT CGT GAG AAC CTC CTT CGT GGT CTT AAG CCT TTA CTT AGT AGA    TCA TCC TTA TCA TCA TCT TCT TTC ATT AAA   AGT CAT CCG GGG AGT AGG AAT AGT AGT AGA AGA AAG TAA TTT TCA GTA GGC CCC
RD42	AG CTT ATG GGA GCA CTC TTG GAC TTC ATT AAA AGT CAT CCG GGG       A TAC CCT CGT GAG AAC CTC AAG TAA TTT TCA GTA GGC CCC
RD57	GAT CCT GTG GAG CTG CTT CTA CGA CTA CTG ATA CCT     GA CAC CTC GAC GAA GAT GCT GAT GAC TAT GGA AT
RD58	GAT CCT CGG CAC AAA CCT CAT CTG AAA CCT      GA GCC GTG TTT GGA GTA GAC TTT GGA AT
RD59	TAC GCC TAC AGA CAA GAT AGC CG    G CGG ATG TCT GTT CTA TCG GCA GCT