Table III.
GFP-DAL82 complementation of dal82Δ
| Strain | Plasmid description |
β- Galactosidase activity |
|
|---|---|---|---|
| Proa | Pro + OXLU |
||
| Miller units | |||
| STCY32 (WT) | 1946 | 4684 | |
| SS200 (dal82Δ) | 201 | 139 | |
| SS200 (dal82Δ) | Vector (YCp50) | 233 | 152 |
| SS200 (dal82Δ) | GFP vector (p423) | 231 | 167 |
| SS400 (dal82Δ) | Full-length Dal82p (pSS20) | 1874 | 4834 |
| SS200 (dal82Δ) | GFP-Dal82p (pSS423–82) | 2839 | 5646 |
a
Pro ; OXLU, oxalurate.
WT STCY32 and dal82 SS200 were transformed with DAL7-lacZ reporter pHY43–1 (26); pSS20 (DAL82 under its own promoter), YCp50, or pSS423–82 was used for a second transformation. β-Galactosidase was assayed in transformants grown (split culture) in medium containing 2% minimal glucose, 0.1% proline, and yeast nitrogen base without amino acids or NH4 ± inducer, oxalurate (66 mg/liter). Note that the vector used for microscopy (GAL1,10) and complementation (ADH1) differed by the promoter driving GFP-DAL82 because the strains used for the experiments were of necessity different; dal82 mutant SS400 does not use galactose as sole carbon source.