Table 2. Nucleotide sequences of primers used in this study.
Primer name | Target gene | Nucleotide sequence (5' to 3') | Product size (bp) | Reference |
---|---|---|---|---|
KPC-F | KPC-type | GTCACTGTATCGCCGTCTAGTTC | 909 | This study |
KPC-R | TGGTGGGCCAATAGATGATT | |||
GES-F | GES-type | CGCTTCATTCACGCACTATT | 855 | 20 |
GES-R | GTCCGTGCTCAGGATGAGTT | |||
IMP-1F | IMP-1-type | AAGGCGTTTATGTTCATACTTCG | 605 | This study |
IMP-1R | TTTAACCGCCTGCTCTAATGTAA | |||
VIM-2F | VIM-2-type | ATCATGGCTATTGCGAGTCC | 749 | 21 |
VIM-2R | ACGACTGAGCGATTTGTGTG | |||
NDM-F | NDM-type | GCCCAATATTATGCACCCGG | 738 | This study |
NDM-R | CTCATCACGATCATGCTGGC | |||
OXA-48F | OXA-48-type | GATTATCGGAATGCCTGCGG | 845 | 20 |
OXA-48R | CTACAAGCGCATCGAGCATCA |
KPC, klebsiella pneumoniae carbapenemase; F, forward; R, reverse; GES, greece extended-spectrum β-lactamase; IMP, imipenemase; VIM, Verona imipenemase; NDM, New Delhi metallo-β-lactamase; OXA, oxacillinase.