Fig. 2.
DNaseI protection of sequences required for DAL5 gene expression. Lanes F and G contain the separated products of the Maxam–Gilbert G and A+G reactions. Lanes A–E and H–L contain the digestion products of the 106-bp XhoI EcoRI fragment following incubation without added extract (lanes)- or with increasing amounts of wild-type (strain M970) extract (concentrations in micrograms, numbers at the top of each panel) and limiting amounts of DNasel.