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. Author manuscript; available in PMC: 2015 Apr 3.
Published in final edited form as: FEMS Yeast Res. 2004 Oct;5(1):29–41. doi: 10.1016/j.femsyr.2004.06.004

Fig. 5.

Fig. 5

Electrophoretic-mobility shift assay employing DNA probes derived from the DAL5 promoter region. Left panel, lane A and right panel, lane G contained no protein extract (-Extract). All remaining lanes contained 30 μg of extract prepared from strain W303-1A. The radioactive DNA probes as well as the wild-type (right panel, lanes A–F) and mutant (right panel, lanes H–M) competitor DNA fragments were prepared as described in Section 2. They were added to the EMSA reaction mixtures in the amounts (in μg) indicated. Identities of the two weak complexes are not known.