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. Author manuscript; available in PMC: 2015 Apr 3.
Published in final edited form as: FEMS Yeast Res. 2004 Oct;5(1):29–41. doi: 10.1016/j.femsyr.2004.06.004

Fig. 9.

Fig. 9

β-Galactosidase production supported by plasmids containing synthetic DAL5 fragments covering positions −398 to −335. The synthesized DNA fragments were cloned into heterologous expression vector pNG15, and are the same ones used earlier to identify the bases required for NCR-sensitive gene expression [26]. The extent of the deletions carried in the plasmids is indicated in the figure. DNA was deleted in a symmetrical manner beginning with the first deletion of the 5 bases, TGCCG at positions −370 to −366. The transformation recipient was wild-type RH218 which was grown in minimal γ-aminobutyrate (GABA) medium prior to enzyme assay.