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. Author manuscript; available in PMC: 2016 Jan 10.
Published in final edited form as: Methods Enzymol. 2015 Jan 10;554:81–99. doi: 10.1016/bs.mie.2014.11.012

Figure 6.

Figure 6

Detection of CSE-produced H2S using CLSS-2. Conditions: absence of enzyme (white), 10 µg CSE (light gray), 20 mM Hcy, 20 mM BCA, 25 µM pyruvate, 25 µM NH3; incubated at in 3.0 mL buffer at 37 °C for 48 h prior to detection with 50 µM CLSS-2. Comparison with C6 cell lysates containing 2 × 106 cells (dark gray). Each data point represents the mean ± SE derived from at least three independent experiments; *p<0.005, **p<0.001. Reprinted with permission from Bailey and Pluth (2013). Copyright 2013 American Chemical Society.