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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Cell. 2015 Jan 8;160(0):105–118. doi: 10.1016/j.cell.2014.12.005

Figure 3. MRTFA and SRF overexpression prevent differentiation of MSCs to adipocytes.

Figure 3

(A and B) qPCR and Western blot analysis of indicated mRNAs (A) and proteins (B) in C3H/10T1/2 MSCs undergoing brown adipogenesis.

(C) C3H/10T1/2 cell lines ectopically expressing SRF or MRTFA were induced to differentiate then visualized by phase contrast microscopy.

(D and E) The cell lines (C) were differentiated in absence (D) or presence (E) of BMP7 and isolated RNA subjected to qPCR analysis. See also Figure S3.