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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Cell. 2015 Jan 8;160(0):105–118. doi: 10.1016/j.cell.2014.12.005

Figure 5. Cells from the stromal vascular fraction (SVF) of MRTFA–/– ING WAT express less smooth muscle-like features and undergo brown adipogenesis more extensively than WT SVF cells.

Figure 5

(A) Western blot analysis on BAT and WAT enriched proteins of adipocytes arising from hormonal induction of SVCs isolated from WT and MRTFA–/– inguinal depots.

(B) WT and MRTFA–/– SVC adipocytes were treated with or without forskolin (FSK) for 4 h prior to isolation of total RNA for analysis. Values are presented as fold change in response to treatment with FSK in WT and MRTFA–/– adipocytes.

(C and D) Subconfluent SVCs from ING and EPI WAT of WT and/or MRTFA–/– mice were exposed to TGFβ1 or BMP7 for 2 d before reaching confluence and subjected to Western blot analysis.