(A) Western blot analysis on BAT and WAT enriched proteins of adipocytes arising from hormonal induction of SVCs isolated from WT and MRTFA–/– inguinal depots.
(B) WT and MRTFA–/– SVC adipocytes were treated with or without forskolin (FSK) for 4 h prior to isolation of total RNA for analysis. Values are presented as fold change in response to treatment with FSK in WT and MRTFA–/– adipocytes.
(C and D) Subconfluent SVCs from ING and EPI WAT of WT and/or MRTFA–/– mice were exposed to TGFβ1 or BMP7 for 2 d before reaching confluence and subjected to Western blot analysis.