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. Author manuscript; available in PMC: 2015 Apr 3.
Published in final edited form as: J Biol Chem. 2004 Feb 16;279(18):19294–19301. doi: 10.1074/jbc.M309240200

Fig. 11. Gln3 localizes to projections emanating from the nucleus during its exit from and entry into the nucleus.

Fig. 11

Cultures (TB123) were either grown to mid-log in YNB-glutamine and transferred to proline medium for 30 min (rows A–E) or YNB-proline and transferred to glutamine for 1 min (row F); images in row F were taken from Cox et al. (7) for comparison. Cells were then processed for indirect immunofluorescence. The localization of Gln3-Myc13 and nuclei was determined by staining with monoclonal antibody 9E10 anti-Myc (columns 1 and 2) and DAPI (column 2, blue), respectively. Merged images (column 2) show the overlap (pink) of Gln3 and nuclei. Micrographs were imaged as described in Fig. 9.