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. Author manuscript; available in PMC: 2015 Apr 3.
Published in final edited form as: J Biol Chem. 2004 Feb 16;279(18):19294–19301. doi: 10.1074/jbc.M309240200

Fig. 5. Cell cycle arrest does not inhibit Gln3-Myc nuclear accumulation when cells are transferred from a good nitrogen source to a poor one.

Fig. 5

A yeast culture (TB123) was grown to log phase (A600 nm = 0.2) in YNB-ammonia medium. Half of the culture was pretreated with 200 mM hydroxyurea for 2 h at 30 °C (+OH-urea). 70–80% of the cells were scored as large budded cells after this incubation, demonstrating that cell cycle arrest had been achieved. Aliquots were removed just before transfer to YNB-proline (0 min) and at 30 and 60min after transfer and processed for immunofluorescence.