Fig. 7. An intact actin cytoskeleton is not required for the cytoplasmic accumulation of Gln3.

Cultures (TB123) were grown to mid-log in 2% glucose, YNB (without amino acids or ammonium sulfate), and 0.1% proline and treated with latrunculin B as described in Fig. 2. Aliquots were then removed just before transfer from proline to glutamine medium (0 min) and at the indicated times after transfer and processed for indirect immunofluorescence.