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. Author manuscript; available in PMC: 2015 Apr 3.
Published in final edited form as: J Biol Chem. 2004 Feb 16;279(18):19294–19301. doi: 10.1074/jbc.M309240200

Fig. 8. An intact actin cytoskeleton is not required for the nuclear localization of Gln3 after rapamycin treatment.

Fig. 8

Cultures (TB123) were grown to mid-log in 2% glucose, YNB (without amino acids or ammonium sulfate), and 0.1% ammonium sulfate and pretreated with either 150 μM latrunculin B (+LAT) or the carrier, ethanol (−LAT) as described in Fig. 2. Aliquots were then removed just before the addition of 200 ng/ml rapamycin (0 min) and at the indicated times after the addition. Samples were processed for indirect immunofluorescence as in Fig. 2.