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. Author manuscript; available in PMC: 2015 Apr 3.
Published in final edited form as: J Biol Chem. 2004 Feb 16;279(18):19294–19301. doi: 10.1074/jbc.M309240200

Fig. 9. Intracellular localization of Gln3 and actin.

Fig. 9

Cultures (TB123) were grown to mid-log in 2% glucose, YNB-glutamine medium (rows A and B) or were transferred from glucose-ammonia to -proline medium, incubated at 30 °C for 22 min (rows C and D), and processed for indirect immunofluorescence. The localization of actin, Gln3-Myc13, and nuclei was determined by staining with phalloidin (columns 1 and 3, green), monoclonal antibody 9E10 anti-Myc (columns 2–4, red), and DAPI (column 4, blue), respectively. Merged images (column 3) show the overlap (column 3, yellow) of Gln3 and actin or Gln3 and DAPI-positive material (column 4, pink). Micrographs were imaged using a Zeiss Axioplan 2 imaging microscope. 0.4-μm sections were collected as a Z-stack, and one image from the center of that stack is shown. Images were deconvolved with AxioVision 3.0 software using the constrained iterative algorithm.