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. Author manuscript; available in PMC: 2015 Apr 3.
Published in final edited form as: Bone. 2014 Jan 23;61:125–131. doi: 10.1016/j.bone.2014.01.004

Fig. 1.

Fig. 1

Real-time RT-PCR analysis of autophagy marker Atg5, LC3 and Atg16L mRNA expression. Ground based control (Xg) and microgravity (μXg) subjected mouse bone marrow non-adherent cells were treated with or without RANKL (75 ng/ml) for 24 h. Total RNA isolated were subjected to real-time RT-PCR analysis using gene specific primers for (A) Atg5, (B) LC3 and (C) Atg16L. The mRNA expression was normalized with respect to GAPDH amplification. Each bar represents the mean ± SD of three independent experiments. *Significant (P < 0.05) difference when compared to ground based control without RANKL treatment.