Fig. 5.
Autophagy inhibitor (3-MA) inhibits μXg elevated OCL formation. Mouse bone marrow derived non-adherent cells were cultured with RANKL (75 ng/ml) and M-CSF (10 ng/ml) for 7 days in the presence and absence of 3-MA (2 mM) under Xg and μXg conditions. TRAP-positive multinucleated osteoclasts formed at the end of the culture period were scored. *Significant (P < 0.05) difference when compared to ground based control.