Figure 2. Auxin effect on vacuoles precedes cell size regulation.
(A and B) Time course imaging of 250 nM NAA treated seedlings were performed every 15 min. Image acquisition took 10 min per time point. Graphs depict vacuolar morphology index (A) and cell length of atrichoblasts (B). Untreated seedlings were imaged before and after recording the auxin treated samples and resulting average was defined as T0. Error bars represent s.e.m. For statistical analysis DMSO and NAA treatments were compared. n = 50 cells in 10 individual seedlings for each time point. Student's t-test p-values: *p < 0.05 **p < 0.01, ***p < 0.001.
Figure 2—figure supplement 1. Auxin effect on vacuolar morphology increases in time.
Auxin (250 nM NAA) induced changes in vacuolar morphology over time. Error bars represent s.e.m. For statistical analysis DMSO and NAA treatments were compared. n = 50 cells in 10 individual seedlings for each time point. Student´s t-test p-values: ***p < 0.001.