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. Author manuscript; available in PMC: 2015 Dec 11.
Published in final edited form as: Adv Healthc Mater. 2014 Nov 5;4(4):534–539. doi: 10.1002/adhm.201400410

Figure 2.

Figure 2

Statistical designs of experiments approach was used to (A) investigate the interactions between RGD, YIGSR and IKVAV adhesion peptides (0–100 μM each) and their ability to promote cell survival. Experiments give a (B) predictive max peptide mixture and (C) the effect magnitude of all factors and two-factor interactions. Confirmation of (D) max and min predictive peptide mixture. (E–G) A second series of optimization experiments to test higher peptide concentrations for RGD and IKVAV (100–200 μM each). (H–J) A third series of optimization experiments to test higher peptide concentration for IKVAV (200–300 μM). (K) Validation of the three optimized conditions compared to RDG, RGD and 100 μM of each peptide. Phase images of the (L–N) three optimized conditions with spread cells outlined. Scale bar = 100 μm.