Figure 3. Enforced expression of BTK in myeloma cells confers features of stemness.

(A) Immunoblot analysis of BTK^OE and BTK^WT ARP1 and OPM2 myeloma cells.

(B) Bar diagram of qPCR measurements indicating elevated expression of 4 stemness genes in BTK^OE compared to BTK^WT cells.

(C) Shown to the left are the percentages of SP (indicated in circle) in ARP1-BTK overexpressing cells gated based on verapamil treated cells. The bar graph to the right depicts the fold increase in the SP fraction in BTK^OE vs. BTK^WT samples.

(D) Time course of tumor growth in NOD-SCID mice indicating that BTK^OE cells are more aggressive than BTK^WT cells.