(A) Flow histograms of eFluxx dye retention assays using BTKOE ARP1 and OPM2 cells treated with CGI1746.
(B) Bar diagram of colony formation results indicating CGI1746-dependent reductions in clonogenicity of GFPHighBTKHigh vs. GFPLowBTKLow myeloma cells.
(C) Kaplan-Meier survival curve (left) of C57BL/KaLwRij mice treated with IV injections of either SP or non-SP 5TGM1 cells, followed by treatment of mice with CGI1746 or solvent control and the right panel is a graph of mean serum IgG2b levels of tumor-bearing mice.
(D) Column diagram presenting results of colony formation assays of hematopoietic stem cells (HSC) obtained from BTK-deficient Xid or normal mice.
(E) Working model on the putative mechanisms by which BTK promotes stemness and drug resistance in myeloma.