Fig. 1. Experimental design. Treatments were administered by intraperitoneal injection once per day. Group T1 was administered saline vehicle for 4 weeks; group T2 was administered 10 mg methyl-6-(phenylethynyl)-pyridine (MPEP)/kg body weight for 4 weeks. MPEP was re-administered 2 and 4 weeks later, followed by behavioral analysis 30 minutes later. BTBR, BTBR T+tf/J.