Fig. 6. Northern blot analysis of steady-state CAN1 mRNA profiles when upstream GATA sequences are mutated.
RNA was isolated from wild type (W.T., lanes A, B, and I) or can1Δ cells transformed with pRR467 (lanes C, D, and J), pRR465 (lanes E, F, and K) or pRR478 (lanes G, H, and L) grown in either minimal glucose-proline (PRO) or minimal glucose-glutamine (GLN) medium and hybridized with a CAN1 probe (+22 to +1348). The blot containing lanes I–L is provided to facilitate a more direct comparison of the sizes of the CAN1 mRNA species from cells grown in minimal glucose-glutamine (GLN) medium.
